Dye for Mindray 5-Part Hematology Analyzers erimu langi ez’enjawulo eza polymethine ne nucleic acid fluorescent ezikwatagana mu ngeri ey’enjawulo ku DNA ne RNA y’obutoffaali. Ebirungo bino biwandiika obutoffaali bw’omusaayi obweru, reticulocytes, ne platelets munda mu katoffaali akakulukuta, ne kisobozesa okwawula scattergram mu bitundu bingi nga bayita mu kusaasaanya ekitangaala kya semiconductor laser light scatter n’okuzuula fluorescence.
Ebirungo ebikola amabala / langi eby’omusaayi (Hematology Stain / Dye Reagents) bye biruwa?
Hematology stain/dye reagents for automated analyzers ze ddagala ezirimu ebirungo ebiyitibwa organic fluorophores, surfactants, ne osmotic stabilizers. Okwawukana ku mabala agasiiga omusaayi mu ngalo microscopic, bino liquid dye reagents bikola munda mu closed-fluidic automated flow cytometry systems.
Nga efuyiddwa mu kisenge ky’ensengekera y’okuzuula, langi eyitira mu bitundu by’obutoffaali ebitegekeddwa ebirungo bya lyse ebitongole era n’ekwatagana mu ngeri ya stoichiometric ku nucleic acids ez’omu butoffaali. Bwe biba biweereddwa ekitangaala kya layisi ekya semikondokita munda mu katoffaali akakulukuta, ebiwujjo ebisibiddwa bifulumya ekitangaala ekitangalijja eky’ebbali ekigeraageranye n’ekirimu asidi wa nyukiliya mu butoffaali.
Omulimu mu Kugabanya / Okuzuula Obutoffaali
Fluorescent dye reagents zisobozesa okwawula mu maaso mu bungi bw’obutoffaali bw’omusaayi obuzibu:
Okuwandiika ku Nucleic Acid:Fluorophores ziyingira mu miguwa gya RNA ne DNA egy’omu butoffaali. Obutoffaali obulina emirimu gy’okukyusakyusa emmere egy’amaanyi oba emitendera egitakuze bunyiga ebirungo bya langi ebingi, ne bivaamu obubonero obw’amaanyi obw’ekitangaala.
Omulembe gwa Scattergram:Okugatta ekitangaala eky’okusaasaanya ku mabbali n’amaanyi g’okumasamasa okw’ebbali kitondekawo okusaasaana kw’ebipimo ebingi-.
Okukebera Obutoffaali mu ngeri etaali ya bulijjo:Distinct fluorescent emission profiles zawula atypical lymphocytes, immature granulocytes, n’obutoffaali obumyufu obulina nucleated okuva mu butoffaali obweru obukuze.
Emikutu gy’okuzuula
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Omukutu gw'okuzuula |
Erinnya ly'omukutu / Ekigendererwa |
Enkola y’ebiramu & Enkola y’okupima |
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Omukutu gwa WNR |
Omukutu gwa White Cell Nucleated & RBC |
Apima omuwendo gwa WBC gwonna era n’abala NRBC. Basophils zaawulwamu nga ziyita mu kusaasaana kw’ebintu ate NRBC nucleic acids ziwandiikibwako mu ngeri ya fluorescent. |
|
Omukutu gwa WDF |
Omukutu gw’enjawulo ogw’obutoffaali obweru |
Yawula Neutrophils, Lymphocytes, Monocytes, ne Eosinophils. Epima Granulocytes ezitakuze okuyita mu maanyi g’okusiba langi ya nucleic acid fluorescent. |
|
Omukutu gwa WPC |
Omukutu gwa White Cell Precursor |
Okukebera obutoffaali bwa jjajja, lymphocytes ezitali za bulijjo, n’okubwatuka. Akozesa enkola ey’enjawulo ey’okuyita mu lususu (specialized membrane permeability reaction) nga egattibwa wamu n’okutwala langi okwawula obutoffaali bwa blast. |
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Omukutu gwa RET |
Omukutu gwa Reticulocyte |
Epima omugerageranyo gwa Reticulocyte, omuwendo omutuufu, Immature Reticulocyte Fraction, ne Red Blood Cell equivalent parameters okuyita mu RNA fluorescent staining. |
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Omukutu gwa PLT |
Omukutu gw’amaaso ogwa Platelet |
Ewa okubala obutoffaali obutono (optical platelet counting) nga ekozesa okuwandiika langi ya fluorescent ku platelet RNA, okuziyiza okutaataaganyizibwa okuva mu microcytic RBCs oba ebisasiro. |
Omulongooti gwa Analyzer ogukwatagana
Omusomo gw’okujjanjaba omusaayi mu layisi ogw’ebitundu 5 mu ngeri ey’obwengula:Ekoleddwa ku nkola z’amazzi nga ekozesa semiconductor laser fluorescence flow cytometry.
High-Ebifo eby'okukoleramu eby'okujjanjaba omusaayi mu ngeri ya Modular Hematology:Ekwatagana ne automated cap-piercing samplers ne layini z’amazzi ezitikka obutasalako.
Integrated Hematology-Enkola z'okukola slide:Ekakasiddwa ku nkola z’okutuusa reagent obutereevu mu layini awatali kuwuguka kwa siginiini oba enkyukakyuka ya puleesa.
Ebikwata ku Bikozesebwa
|
Parameter |
Omutindo gw'ebyekikugu / Data |
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Ekika ky’Ebikola |
Ekisengejjo kya langi ya fluorescent amazzi amangi |
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Omusingi gwa Fluorophore ogukola |
Polymethine / langi ya nucleic acid eyakaayakana |
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Obuwanvu bw’amayengo ag’okusikirizibwa |
633 nm – 635 nm (Layisi ya semikondokita emmyufu) . |
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pH Range (diguli 25 ) . |
7.00 – 8.50 |
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Obutambuzi (diguli 25 ) . |
1.20 – 2.50 mS/cm |
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Densite (diguli 20 ) . |
1.005 – 1.020 g/cm3 |
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Open-Okutebenkera kw'Ekiveera |
Ennaku 60 (diguli 2 – diguli 30 mu kutereka mu nzikiza) |
|
Obulamu bw’Etterekero Obutagguddwawo |
Emyezi 18 |
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Ebbugumu ly’okutereka |
2℃– 30℃(Kuuma okuva ku kitangaala n’okutonnya) |
|
Okusengejja obutundutundu |
Esengejebwa okuyita mu 0.2 μm membrane |
Okukozesa & Omulimu gwa Reagent
Okwekenenya enjawulo mu WNR:Efuyiddwa mu kisenge kya WNR reaction okuwandiika ku butoffaali obumyufu obulina nucleus ne nuclei z’obutoffaali obweru oluvannyuma lw’okusaanuuka kwa cytoplasm.
WDF 5-Ekitundu WBC Okwawula:Ekolagana n’ebitundu bya WBC mu mukutu gwa WDF, n’ekola ebibinja eby’enjawulo eby’ekitangaala (fluorescence clusters) ku lymphocytes, monocytes, neutrophils, ne eosinophils.
Okussaako bendera y’obutoffaali obusookerwako & okubwatuka:Yakolebwa okulonda okuyingira mu bitundu by’amasavu ebikyusiddwa mu mukutu gwa WPC, okusobozesa okuzuula ebika by’obutoffaali obutakuze.
Okugera obungi bwa RNA ya Reticulocyte:Ekwatagana ne cytoplasmic ribosomal RNA mu reticulocytes, okusobozesa okubala emitendera gy’okukula kw’obutoffaali obuyitibwa reticulocyte.
Okubala obutoffaali obukola omusaayi mu maaso:Amabala ebitundu bya RNA eby’omunda eby’obutoffaali bw’omusaayi okukola okubala kw’amaaso wansi w’embeera z’obujjanjabi ez’obutoffaali obutono-.
Package / Okutereka
|
Ekintu |
Okunnyonnyola |
|
Okupakinga kwa mutindo |
12 mL x 1 eccupa / bbokisi, 42 mL x 1 eccupa / bbokisi |
|
Ebikozesebwa mu ccupa |
Eccupa za HDPE eziriko fluorine eza amber eziriko engabo ekitangaala- |
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Ebbugumu ly’okutereka |
diguli 2 okutuuka ku diguli 30 |
|
Ebyetaago by’entambula |
Kuuma obutafuuka bbugumu n’omusana obutereevu |
Ebikozesebwa mu kukebera omusaayi (Hematology Reagents).
Diluent Solution for 5-Part Hematology Analyzers: Okukebera omusaayi mu bitundu 5:Isotonic conductive buffer solution okukendeeza ku sampuli y’omusaayi n’okuziyiza amaloboozi ag’emabega.
Lyse Reagent for 5-Part Hematology Analyzers: Okukebera omusaayi mu bitundu 5:Surfactant-based lysing solution okusobola okulonda okusaanuuka kw’olususu lw’obutoffaali obuyitibwa erythrocyte.
Ekizibu ky’okwoza ekizigo:Ekyuma ekiyonja amazzi okusobola okuddaabiriza layini y’amazzi mu ngeri ey’otoma n’okuggyawo ebitereke bya puloteyina.
Ekyuma ekiyonja Probe:Alkaline hypochlorite probe okunaaba ekirungo okusobola okuggyamu puloteyina mu sampuli probe.
Ebibuuzo ebibuuzibwa
Q: Ekirungo kya langi ekitangalijja ekya automated fluorescent dye reagent kyawukana kitya ku bikozesebwa eby’ekinnansi eby’okusiiga langi mu musaayi?
A: Amabala ag’ekinnansi agasiiga geetaaga okukala mu ngalo ku slayidi z’endabirwamu okusobola okwekenneenya okulaba wansi wa microscope. Automated analyzer dye reagents ze langi ezimasamasa ez’amazzi ezikoleddwa mu bisenge by’ensengekera eziri mu layini. Zisiba ku RNA/DNA y’obutoffaali mu kuyimirizibwa kw’amazzi, ne zifulumya obubonero bw’ekitangaala obuzuuliddwa laser flow cytometry optics.
Q: Okukwatibwa ekitangaala kikosa enkola ya langi mu kiseera ky’okukola buli lunaku?
A: Yee. Fluorophores ezikola zivunda wansi w’okubeera mu kitangaala ekitereevu oba ekitangaala kya UV ekibeera mu kifo ekiwanvu. Ebisengejja bipakiddwa mu bidomola bya amber ebikuumibwa ekitangaala-era era birina okusigala munda mu kisenge ky’ebirungo ebiggaddwa nga bikola.
Q: Bbugumu ki ery’okutereka eryetaagisa mu kiseera ky’okutambuza n’okutereka?
A: Teeka ebirungo ebikola ebirungo wakati wa diguli 2 ne diguli 30 . Okutereka wansi wa diguli 0 kireeta okutonnya kwa fluorophore. Ebbugumu erisukka diguli 35 lyanguya okuvunda kw’ebirungo ebikola.
Q: Okukwatagana kwa lot-to-lot kukwata kutya ku bivudde mu scattergram?
A: Enjawulo mu bungi bwa langi ereeta enkyukakyuka mu bibinja bya scattergram, ekivaako ensobi mu gating. Buli kifo ekifulumya kiyita mu kukakasa obulongoofu bwa fluorophore, okugezesebwa kwa spectrofluorometric, n’okukwatagana kwa matrix okukuuma ekifo ekikwatagana ekya scattergram.
Q: Dizayini ki ey’okupakinga ekozesebwa okuyungibwa mu mazzi?
A: Ebirungo ebikola langi biweebwa mu bucupa bwa HDPE obusibiddwako fluorinated nga buliko cap adapters ezikoleddwa ku layini eziyingiza amazzi obutereevu, okuziyiza okufuumuuka n’obucaafu.
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