Reticulocyte Dye For Sysmex Okukebera Omusaayi

Reticulocyte Dye for Sysmex Hematology Analyzers egaba okusiiga kwa nucleic acid okutunuuliddwa ku semiconductor laser flow cytometry platforms. Ekirungo ky’amazzi kiyingira mu butoffaali bw’omusaayi obulina nucleated, reticulocytes, ne platelets okusiba mu ngeri okulondamu ne RNA ne DNA mu butoffaali, ne kikola obubonero obw’enjawulo obw’okumasamasa ku mabbali n’okusaasaanya ekitangaala okuyita mu mikutu egyalagirwa egy’okuzuula. Ekoleddwa okugatta obutereevu n’enkola z’amazzi ez’obwengula, eky’okugonjoola kikuuma amaanyi g’ekitangaala ekinywevu, amaloboozi amatono ag’emabega, n’okukola kwa lot-to-lot eziyinza okuddamu mu mbeera za laboratory ez’obujjanjabi.

Automated hematology dye reagents ze mazzi fluorescent markers ezikoleddwa yinginiya okukola flow-cytometry-based cell analysis platforms. Okwawukanako n’amabala ag’ennono ag’okusiiga omusaayi mu ngalo agakozesebwa mu microscopy y’ekitangaala, langi zino ez’amazzi ziyingira mu butoffaali obugendererwamu nga ziyita mu controlled membrane permeabilization okuwandiika intracellular nucleic acids. Wansi w’okusikirizibwa kwa layisi ya semikondokita munda mu katoffaali k’okutambula kw’amaaso, ensengekera z’obutoffaali eziwandiikiddwa zifulumya obubonero bw’okumasamasa okw’ebbali n’okusaasaana kw’ekitangaala, okusobozesa enkola y’amaaso okukola scattergrams ezituufu ez’ebitundu bibiri- n’ensaasaanya y’ebibinja by’obutoffaali.

Mu kwekenneenya omusaayi ogw’amaaso, langi ezimasamasa zisobozesa okusosola kw’obutoffaali obw’enjawulo nga zigera obungi bw’obungi bwa nucleic acid mu butoffaali:

Enjawulo mu butoffaali bw’omusaayi obweru:Yawula lymphocytes, monocytes, neutrophils, eosinophils, ne basophils okusinziira ku migerageranyo gya RNA/DNA n’ensengekera ya cytoplasmic.

Okuzuula Obutoffaali obutali bwa bulijjo & obutakula:Azuula obutoffaali bwa blast ne granulocytes ezitakuze okuyita mu maanyi g’okufulumya ekitangaala ekigulumivu.

Okubala obutoffaali obuyitibwa Reticulocyte:Epima RNA ya ribosomal esigadde mu butoffaali obumyufu obuto okwekenneenya emirimu gy’okukola erythropoietic mu busimu bw’amagumba.

Okubala obutoffaali obukola omusaayi mu maaso:Egonjoola obutoffaali obunene obuyitibwa platelets n’okwawula ebitundu by’obutoffaali obumyufu ku platelets ng’ekozesa target nucleic acid fluorescence gating.

Ddayi ya fluorescent ekola mu mikutu egy’enjawulo egy’amaaso egy’obwengula:

Omukutu gwa WNR:Abala obutoffaali obweru n’okwawula obutoffaali obumyufu obulina nucleus okuyita mu differential nucleic acid staining.

Omukutu gwa WDF:Yawula ebitundu bya leukocyte ebikuze era n’ezuula granulocytes ezitakuze nga ekozesa side fluorescence ne side scatter parameters.

Omukutu gwa WPC:Okukebera obutoffaali bwa jjajja n’obutoffaali obutali bwa bulijjo nga kwesigamiziddwa ku density ya nuclear n’okuyita kw’olususu olw’enjawulo.

Omukutu gwa RET:Epima obutundutundu bwa reticulocyte n’ebipimo by’obutoffaali obumyufu obw’amaaso okuyita mu maanyi ga RNA fluorescence.

Omukutu gwa PLT:Awandiika ku asidi za nucleic eziyitibwa platelet nucleic acids okumalawo okutaataaganyizibwa kw’obutoffaali obumyufu obuyitibwa microcytic red blood cell interference mu kiseera ky’okubala mu maaso.

Ekoleddwa okusobola okukwatagana obutereevu emirimu n’emikutu gy’omusaayi egy’obwengula nga ekozesa semiconductor laser flow cytometry:

XN Series:XN-1000, XN-2000, XN-3000, XN-9000, XN-L Omusinde (XN-350, XN-450, XN-550) Enkola ya XN-L.

XS Series:XS-500i, XS-800i, XS-1000i

XT Series:XT-1800i, XT-2000i, XT-4000i

XE Series:XE-2100, XE-5000

Okusiiga emikutu gya WNR (STAC-DYE-SYSWNR):Permeabilizes cell membranes okusobozesa okuyingira amangu kwa langi ya nuclear, okwawula nuclei za NRBC ezitaliiko kamogo okuva ku butoffaali obweru okusobola okukola NRBC % entuufu n’omuwendo gwa WBC.

Okusiiga emikutu gya WDF (STAC-DYE-SYSWDF):Amabala ku nucleic acids mu butoffaali eza leukocytes okukola ebibinja eby’enjawulo ebya scattergram ku neutrophils, lymphocytes, monocytes, ne eosinophils.

Okusiiga emikutu gya WPC (STAC-DYE-SYSWPC):Okulonda okusiiga obutoffaali bwa blast ne lymphocytes ezitali za bulijjo okusinziira ku lipid composition ne nuclear density for secondary cell flagging.

RET Okusiiga emikutu (STAC-DYE-SYSRET):Yesiba ku RNA esigadde mu butoffaali obumyufu obutakuze, okusobozesa okubala ebitundu bya reticulocyte byonna awamu, omuwendo omutuufu, n’ekitundu kya reticulocyte ekitali kikuze.

Okusiiga emikutu gya PLT (STAC-DYE-SYSPLT):Awandiika ku asidi za nucleic za platelet okuziyiza okutaataaganyizibwa kw’obutoffaali obumyufu obuyitibwa microcytic mu kiseera ky’okupima platelet z’amaaso ez’omuwendo omutono-.

Ensengeka z'okupakinga:

• Kaseti za kkatiriji: 2 × 12 mL
• Ekizimbulukusa mu ccupa: 1 × 50 mL, 1 × 100 mL, 1 × 500 mL

Ebbugumu ly’okutereka:Diguli 2 okutuuka ku diguli 30 (Kuuma okuva ku kitangaala ekitereevu n’okutonnya)
Obulamu bw’ebintu ebitagguddwawo:Emyezi 18
Ku-Okutebenkera kwa Board:Ennaku 60 mu mbeera ya ambient laboratory

Ebigonjoola ebiziyiza omusaayi (Hematology Diluent Solutions):Isotonic buffer solutions ezikuuma obulungi bw’obunene bw’obutoffaali n’okufuga obutambuzi bw’amazzi mu kiseera ky’okupima eby’omubiri.
Ebirungo bya Lyse eby’enjawulo mu Leukocyte:Ebisengejja ebikola (active solutions) ebisunsulamu obutoffaali obumyufu okuteekateeka obutoffaali obweru okwekenneenya mu maaso.
Ebikozesebwa mu kwoza Analyzer:Amazzi ag’okunaaba aga enziyiza agakoleddwa okugogola ebizimba bya puloteyina n’ebisigadde bya langi okuva mu layini z’amazzi ez’omunda n’obutoffaali obukulukuta obw’amaaso.
Amazzi g’ekikuta agasengekeddwa:Amazzi agakola amazzi agakoleddwa okutuuka wakati mu migga gya sampuli munda mu bisenge ebizuula eby’amaaso.

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