WPC Dye for Sysmex 5-Part Hematology Analyzers etuusa high-specificity fluorescent staining ku mikutu gy’okuzuula obutoffaali obweru obuzaale mu nkola z’okwekenneenya omusaayi ogw’amazzi agayitamu ennyo. Ekoleddwa olw’oluyoogaano olutono olw’amaaso olw’emabega n’okuyita kw’oluwuzi lw’obutoffaali olw’amangu, ekirungo kino kisobozesa okuyingira mu ngeri entuufu ey’okusaasaana kw’obutoffaali obutono (immature leucocytes) n’ebibinja by’obutoffaali obutali bwa bulijjo mu kiseera ky’okudduka kw’okutambula kw’obutoffaali obutambula mu ngeri ey’obwengula (automated laser flow cytometry runs).

Ebirungo ebikola amabala / langi eby’omusaayi (Hematology Stain / Dye Reagents) bye biruwa?

Automated hematology fluorescent dye reagents ze nsengekera z’eddagala ery’amazzi ezikoleddwa yinginiya w’okubala obutoffaali obw’obwengula obw’amaanyi-speed automated cell counters. Okwawukanako n’amabala agasiigibwa mu musaayi aga microscopic manual, ebisengejjo bino eby’amazzi biyingira mu bisenge by’ensengekera y’amazzi amatonotono, ne biyita mu membranes z’obutoffaali, ne bitunuulira asidi za nucleic eziri mu butoffaali (DNA ne RNA). Okufuluma kw’ekitangaala ekivaamu kisobozesa ensengekera za sensa ez’amaaso okugabanya obungi bw’obutoffaali okusinziira ku bungi bwa asidi wa nyukiliya n’obuzibu bw’ensengekera y’omunda.

Omulimu mu Kugabanya / Okuzuula Obutoffaali

Mu kiseera ky’enzirukanya y’okwekenneenya, langi eno yeekwata mu ngeri elondamu ku miguwa gya nucleic acid egy’omu butoffaali. Bwe bayita mu kitangaala kya layisi ekya semikondokita, ebitundu ebiteekeddwako akabonero bifulumya obubonero bwa fluorescent. Nga ekwata amaanyi g’okumasamasa okw’ebbali ku mabbali g’okusaasaana kw’ekitangaala mu maaso n’ebbali, enkola eno egonjoola ebibinja by’obutoffaali ebizibu enkola z’okuziyiza amasannyalaze eza bulijjo bye zitasobola kwawula, n’ewa okuzuula okwesigika kw’obutoffaali obukuba n’ebisookerwako ebitakuze.

Omukutu gw'okuzuula

Omulimu omukulu ogwa langi eno gwesigamye ku mukutu gwa WPC (White Cell Progenitor), ogwawula obutoffaali obusookerwako okuva mu bungi bw’obutoffaali obukulu.

Omukutu gwa WPC:Fluorescent nucleic acid staining okusobola okwawula obutoffaali bwa jjajja, blasts, ne lymphocytes ezitali za bulijjo.

Omukutu gwa WDF:Enjawulo y’okusiiga ebifaananyi ku neutrophils ezikuze, lymphocytes, monocytes, ne eosinophils.

Omukutu gwa WNR:Enkola y’okuddamu eyeewaddeyo okuzuula obutoffaali obumyufu obuyitibwa nucleated blood cell (NRBC) n’okubala obutoffaali obweru bwonna.

Omukutu gwa RET:Okuwandiika asidi wa nucleic acid okusobola okwekenneenya obuzito bwa reticulocyte n’omuwendo gw’okukula.

Omukutu gwa PLT:Specific fluorescence tagging for high-precision optical platelet counts mu sampuli enzibu.

Omulongooti gwa Analyzer ogukwatagana

Automated 5-Ekitundu ekyekenneenya omusaayi X-Series
Automated 5-Ekitundu ekyekenneenya omusaayi Y-Series
Enkola z’omusaayi mu kutambula kw’omusaayi mu mikutu mingi-

Ebikwata ku Bikozesebwa

Parameter y’ebyekikugu	Omutindo gw’okulaga ebikwata ku nsonga
Ennamba ya Katalogu	STAC-DYE-SYSWPC
Enkula y’Omubiri	Ekizimbulukusa ky’amazzi ekitangaavu
pH Range	6.80 – 7.40 ku diguli 25
Entikko y’okunyiga ($\\ lambda_{max}$) .	635 nm – 645 nm
Omukutu ogugenderera	WPC (Omukulu w’obutoffaali obweru) .
Enkola y’okuzuula	Semiconductor Laser Flow Cytometry (okupima amazzi agakulukuta mu bitundutundu).
Lot-ku-Lot CV	Ebitono oba byenkana 2.5% .
Ku-Okutebenkera kwa Board	Ennaku 60 oluvannyuma-okuggulawo
Obulamu bw’Etterekero Obutagguddwawo	Emyezi 18
Ebbugumu ly’okutereka	2℃– 30℃(Kuuma okuva ku kitangaala)

Okukozesa & Omulimu gwa Reagent

Okussaako Bendera y’obutoffaali bwa Lymphocyte mu ngeri etaali ya bulijjo:Eyita mu nsengekera z’obutoffaali okuwandiika RNA/DNA mu butoffaali, n’ekola ensengekera za scattergram ez’enjawulo ku bungi bwa lymphoid obutali bwa bulijjo.

Okuzuula Obutoffaali bwa Blast:Eteekawo ensalo z’amaanyi g’ekitangaala ekitegeerekeka wakati wa leucocytes ezikuze n’obutoffaali bwa blast obw’omutendera ogusooka-.

Okugera obungi bwa Granulocyte ezitakuze:Kyanguyiza okuyingira okutuufu okwa myelocytes, metamyelocytes, ne promyelocytes munda mu nsengeka y’okupima WPC.

Package / Okutereka

Okupakinga kwa mutindo:12 $\\times$ 12 mL ettaala-katiriji eziriko engabo buli bbokisi.

Enkola z’okugabira abantu ebintu mu bungi:Ensengeka z'ekibinja kya katiriji eziwera-ziriwo ku nkola z'emirimu gya laboratory egy'obwengula egy'obuzito obunene-.

Okutereka n'okutambuza:Teeka mu kifo ekikalu ku diguli 2 – diguli 30 . Kuuma ng’osibye bulungi era ng’okuuma obutakwatibwa kitangaala butereevu. Tofuuka bbugumu. Enywevu wansi w’embeera z’okuyita mu bbugumu ery’omu kifo erya bulijjo.

Ebikozesebwa mu kukebera omusaayi (Hematology Reagents).

SYSWNR Langi ya Fluorescent:Reagent for total white blood cell count n’emikutu gy’amaaso egy’obutoffaali obumyufu obulimu nucleated.
SYSWDF Langi ya Fluorescent:Fluorochrome reagent okusobola okwekenneenya enjawulo ya leucocyte ey’ebitundu 5 ebya bulijjo.
SYSRET Langi etangaaza:Ddayi ya nucleic acid eyetongodde ku nkola z’okupima reticulocyte.
SYSPLT Langi ya Fluorescent:Specific optical stain okusobola okusalawo platelets z’amaaso.

Ebibuuzo ebibuuzibwa

Q: Omulimu gw’ekikugu ogw’omukutu gw’okuzuula WPC gwe guliwa?

A: Omukutu gwa WPC gugatta semiconductor laser flow cytometry ne fluorescent nucleic acid dye okwawula obutoffaali bwa jjajja obusooka, blasts, ne lymphocytes ezitali za bulijjo okuva ku nnyiriri z’obutoffaali obweru obukulu.

Q: Ekirungo kino kyawukana kitya ku mabala aga bulijjo aga microscopic blood smear stains?

A: Amabala aga microscopic geesigamye ku kunyiga langi okusobola okwekenneenya okulaba wansi w’ekitangaala ekitangalijja. Ekintu kino kya mazzi fluorochrome ekyakolebwa yinginiya okusikiriza amazzi mu ngeri ey’obwengula, okuyingira mu luwuzi lw’obutoffaali, n’okuzuula ekitangaala eky’amaanyi-munda mu mikutu gya flow cytometry.

Q: Biki ebikwata ku kutereka n’ekkomo ku kutebenkera kw’emirimu?

A: Ebitundu ebisibiddwa ebitagguddwa bikuuma obutebenkevu bw’eddagala mu bujjuvu okumala emyezi 18 bwe bikuumibwa wakati wa diguli 2 ne diguli 30 mu mbeera ekuumibwa ekitangaala-. Oluvannyuma lw’okutikkibwa ku nkola, ekirungo kino kikuuma omulimu gw’okwekenneenya mu bujjuvu okumala ennaku 60.

Q: Enkola ki ez’okulondoola omutindo ezikakasa obutakyukakyuka bwa lot-to{1}}lot?

A: Buli kitundu ky’okufulumya kiyita mu kukakasa okunyiga kw’entikko mu ngeri ya spectrophotometric, okupima pH, okugezesebwa kw’amaloboozi g’ekitangaala eky’emabega, n’okukakasa enzigi z’obutoffaali obujuliziddwa okukakasa nti omugerageranyo gw’enkyukakyuka (CV) gungi-ku{1}}mu kitundu (CV) wansi wa 2.5%.

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